| Line 1: | Line 1: | ||
| + | |||
| + | For the sequence below: | ||
| + | *Create DNA | ||
| + | *Annotate promoter (-10/-35 sequences) | ||
| + | *Analyze rho independent terminator (ARNold) | ||
| + | *Annotate operator (ttgtgagcggataacaa) | ||
| + | *Analyze RNA 5’ UTR structure (NUPACK) | ||
| + | *Annotate 5’ UTR/3’ UTR | ||
| + | *Locate RBS | ||
| + | *Locate Start/stop codons | ||
| + | ILocate ORF | ||
| + | |||
pT7lacO-GFP sequence | pT7lacO-GFP sequence | ||
| − | * https://benchling.com/s/seq-NlmhOd0p27Kd2K0YMJFA | + | *https://benchling.com/s/seq-NlmhOd0p27Kd2K0YMJFA |
Latest revision as of 12:53, 6 March 2020
For the sequence below:
- Create DNA
- Annotate promoter (-10/-35 sequences)
- Analyze rho independent terminator (ARNold)
- Annotate operator (ttgtgagcggataacaa)
- Analyze RNA 5’ UTR structure (NUPACK)
- Annotate 5’ UTR/3’ UTR
- Locate RBS
- Locate Start/stop codons
ILocate ORF
pT7lacO-GFP sequence