Experiments

14.5.2015 1. PCR of streptavidin gBlock Specs:

• m(tDNA) = 1 ng/reaction
• primers = M13 FWD (-20); M13 FWD (-46)
• cycles = 25
• t(pol) = 50s
• T(gradient) = 50,2°C; 54,6°C; 60,9°C
• polymerase = 95% Taq + 5% Pwo

2. Electrophoresis - 1,5%; 0,8%