Perform the following:
- Create folder structure (sources, fragments, parts, genes)
- Create DNA (duplicate shared sequence below to your sources folder)
- Annotate promoter (-10/-35 sequences)
- Analyze rho independent terminator (ARNold)
- Annotate operator (ttgtgagcggataacaa)
- Annotate 5’ UTR/3’ UTR
- Analyze RNA 5’ UTR structure (NUPACK)
- Locate RBS
- Locate Start/stop codons
- Locate ORF
- translate the ORF
Sequence link