(Created page with " Sequence link *https://benchling.com/s/seq-NlmhOd0p27Kd2K0YMJFA") |
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| − | + | Perform the following: | |
| + | *Create folder structure (sources, fragments, parts, genes) | ||
| + | *Create DNA (duplicate shared sequence below to your sources folder) | ||
| + | *Annotate promoter (-10/-35 sequences) | ||
| + | *Analyze rho independent terminator (ARNold) | ||
| + | *Annotate operator (ttgtgagcggataacaa) | ||
| + | *Annotate 5’ UTR/3’ UTR | ||
| + | *Analyze RNA 5’ UTR structure (NUPACK) | ||
| + | *Locate RBS | ||
| + | *Locate Start/stop codons | ||
| + | *Locate ORF | ||
| + | *translate the ORF | ||
Sequence link | Sequence link | ||
*https://benchling.com/s/seq-NlmhOd0p27Kd2K0YMJFA | *https://benchling.com/s/seq-NlmhOd0p27Kd2K0YMJFA | ||
Latest revision as of 16:29, 6 March 2020
Perform the following:
- Create folder structure (sources, fragments, parts, genes)
- Create DNA (duplicate shared sequence below to your sources folder)
- Annotate promoter (-10/-35 sequences)
- Analyze rho independent terminator (ARNold)
- Annotate operator (ttgtgagcggataacaa)
- Annotate 5’ UTR/3’ UTR
- Analyze RNA 5’ UTR structure (NUPACK)
- Locate RBS
- Locate Start/stop codons
- Locate ORF
- translate the ORF
Sequence link