For the sequence below:

  • Create DNA
  • Annotate promoter (-10/-35 sequences)
  • Analyze rho independent terminator (ARNold)
  • Annotate operator (ttgtgagcggataacaa)
  • Analyze RNA 5’ UTR structure (NUPACK)
  • Annotate 5’ UTR/3’ UTR
  • Locate RBS
  • Locate Start/stop codons

ILocate ORF

pT7lacO-GFP sequence